Code | CSB-MP004953HU |
MSDS | |
Size | $228 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Less than 1.0 EU/ug as determined by LAL method.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
CUSABIO's product CSB-MP004953HU is a recombinant human T-cell antigen CD7. It is produced by the expression of the DNA fragment corresponding to 26-180 AA of the human CD7 protein in mammalian cells. This CD7 protein is fused with a hFc-Myc-tag at the C-terminus. It has been validated to be active through the functional ELISA (bind to the SECTM1, EC50 is 1.236-1.773 ng/ml) and LSPR assay (bind to human SECTM1 protein with an affinity constant of 1.84 nM). The purity of this CD7 protein is greater than 90% measured by SDS-PAGE. It contains less endotoxin, <1.0 EU/ug determined by the LAL method. And it is available now.
CD7 is a cell surface costimulatory molecule expressed on T and NK cells and on cells in the early stages of T-, B-, and myeloid cell differentiation. CD7 binding to its ligand K12/SECTM1 plays a costimulatory role in T-cell activation.
Applications : Binding assay/Protein-protein interaction
Review: I used the CSB-MP004953HU product for an ELISA experiment, and CD7 was detected at 5 μg/ml can bind to human SECTM1, with an EC50 of 1.811-3.372ng/ml. The protein has good binding activity and can be used for my future research and application. It has excellent repeatability, and is convenient to purchase at a favorable price. I plan to purchase in large quantities in the future!
By Anonymous